Luminex assay

Luminex assay is a bead-based immunoassay multiplex technique that allows multiple analytes ex. Generally, the results obtained using a standardized Luminex assay are superior or equivalent to conventional ELISA, luminex assay, even as the differentiated xMAP technology allows high throughput biomarker testing with reduced sample volume, test preparation, and reagent costs. Luminex immunoassays can multiplex luminex assay to compatible analytes simultaneously using a commercial or custom kit.

Premium instruments, software, calibration, and validation kits to ensure consistent and reproducible results with Luminex xMAP assays. Validated Luminex xMAP assays for hundreds of analytes: cytokines, chemokines, signaling, and more. Luminex xMap technology is a bead-based multiplexed immunoassay system in a microplate format. The system can simultaneously detect many targets in a single sample — up to , depending on system design. The microspheres used in the Luminex system have different spectral addresses color codes.

Luminex assay

Attenzione, le pagine di questa sezione sono disponibili solo in lingua inglese. This material is intended for International non-USA site visitors only. Multiplexing is a method for high-volume biomarker testing—or testing multiple analytes simultaneously within a single run—using a single sample volume. Because each microsphere serves as an individual test, a large number of different assays can be performed and analyzed simultaneously. Looking for custom assay design and development services with unique targets in mind? Please select your location below Attenzione, le pagine di questa sezione sono disponibili solo in lingua inglese. International US. Document repository Product documentation. Need more information? Contact us. Need any help? Ask for support. Quick links. How does xMAP Technology work? Generate more data while saving sample, time, and reagents.

Specialties Back Close Menu. Contact us. The biological matrix effect, however, may still be a concern with commercial Luminex immunoassays.

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Premium instruments, software, calibration, and validation kits to ensure consistent and reproducible results with Luminex xMAP assays. Validated Luminex xMAP assays for hundreds of analytes: cytokines, chemokines, signaling, and more. Luminex xMap technology is a bead-based multiplexed immunoassay system in a microplate format. The system can simultaneously detect many targets in a single sample — up to , depending on system design. The microspheres used in the Luminex system have different spectral addresses color codes. These unique spectral addresses are created by internally labeling beads with different ratios of two fluorophores, one in a red wavelength and the other in the far red. This is the xMAP technology.

Luminex assay

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That said, Luminex assays are often a better option since they can multiplex several of these robust single analyte ELISA assays within a single and small-volume biological sample. Luminex xMAP Technology. This material is intended for International non-USA site visitors only. We recommend microspheres per analyte in a Luminex multiplex bead assay to obtain highly accurate and precise results. Contact Us. As needed, we perform sensitivity, selectivity, specificity, matrix effects linearity and spike recovery , inter and intra-day variation, and sample stability to validate your Luminex multiplex assay further. High-throughput testing and analysis Because each microsphere serves as an individual test, a large number of different assays can be performed and analyzed simultaneously. Typically, we utilize this format when the target molecule has a low molecular weight or when only one antibody is available. Furthermore, our scientists would be happy to share a formal audited and hyperlinked pdf report as needed. We highly recommend them and look forward to working together again. Bio-Plex Software : There are a range of software options for system management, calibration and validation, protocol design, data acquisition, data analysis, and data export and reporting.

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VP, Head of Clinical Operations. Luminex Multiplex platform is highly customizable and includes user-friendly software that automatically generates standard curves of known concentrations of all analytes and displays data in graphic or tabular form. In an assay using the Luminex system, the beads are incubated with a sample. NorthEast BioLab tremendously supported us in reproducing our critical lab discoveries for drug metabolism. Bio-Plex assays use antibodies coupled to the beads. As needed, we perform sensitivity, selectivity, specificity, matrix effects linearity and spike recovery , inter and intra-day variation, and sample stability to validate your Luminex multiplex assay further. We have worked with NorthEast BioLab for over ten years given their commitment to highest quality bioanalytical data. That said, we must consider antibody cross-reactivity when designing multiplex experiments using Luminex Assay. This technology can detect multiple targets in a single immunoassay system by assigning each of them with a unique address through the covalent attachment of these fluorescent antibodies to the beads. Luminex Assay: High Throughput Multiplex with Strong Sensitivity and Specificity Luminex immunoassays can multiplex by incubating several pre-coated bead sets with different samples in a microtiter plate. Luminex can facilitate the identification and quantification of multiple biomarker proteins to identify higher-risk patients, determine disease prognosis, or monitor treatment efficacy. Naturally, more extensive multiplex immunoassay studies may take longer due to the larger number of plate runs. Can Luminex Assays multiplex analytes that occur in vastly different biological concentrations e.