Smai cut site

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Enzymes and Inhibitors. Restriction Enzymes. Catalog number: ER Related applications: Restriction Enzyme Cloning. Technical Support Customer Service. Catalog Number. Save to list.

Smai cut site

Hi all, 1 Has anyone used smaI as a RE to cut a vector for blunt end cloning? So my situation now is I'm trying to cut a plasmid bp with smaI. I over digest overnight at room temp. Hope you guys can shed some light SmaI is not the best RE out there, but I understand that sometimes you'd have to use it since it's your only choice. I'd definately phosphatase my plasmid in order to prevent self ligation. I recently used Antarctic Phosphatase and it workes also very good. Good luck!!. Sma I has an isoschizomer which may be a better enzyme. If you still need the blunt end, you can just do a fill-in or chew back the overhang. Hi all, I juz started with cloning in pUC using SmaI to restrict digest for blunt ends and when i ran the gel got a linear plasmid but dint do dephosphorylation

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Enzymes and Inhibitors. Restriction Enzymes. Catalog number: ER Related applications: Restriction Enzyme Cloning. Technical Support Customer Service. Catalog Number.

Smai cut site

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Baldis basics unblocked

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