Spectra viewer

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The SpectraViewer tool enables you to visualize the spectral compatibility of fluorophores and fluorescent labelled probes. Revvity Sites Globally Select your location. How to use the SpectraViewer If you're using a Revvity imaging or detection instrument, start by selecting the instrument from the Select Machine drop-down menu. The light source and filters for the chosen instrument will be displayed automatically. Alternatively, you can choose Custom Instrument or make no selection and add the light sources and filters manually. Light sources are input in nm format and filters as - Next, select the fluorophores of interest from the Add Fluorophore drop-down menu. You may add further fluorophores, light sources and filters at any point, or remove them by clicking X.

Spectra viewer

Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. The continuous development of dyes with improved spectral profiles combined with breakthroughs in light sources, detection methods, and interference filters have paved the way to the adoption of multiplex analysis beyond the realm of flow cytometry. Acquiring large amounts of relevant biological information for each sample has become paramount also in microscopy and imaging experiments. One of the most challenging aspects of multiplex fluorescence analysis is the selection of a combination of fluorochromes with different emission spectra. Spectral overlapping can, in fact, undermine the accuracy and validity of the experiment. It streamlines experiment design and helps to understand which fluorochromes might cause problems, saving troubleshooting time. Pre-loaded light sources and laser sets for microscopy and flow cytometry applications.

If applicable, a list of similar compounds, as well as related categories, will be displayed as well. The choice currently selected will be highlighted in blue. Alternatively, you can choose Custom Instrument or make no selection and add the light sources and filters manually, spectra viewer.

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Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design.

Spectra viewer

Navigation Menu. AAT Bioquest. Cart 0. Sign In. This can be useful for rotating through several overlapping spectra x.

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You may add further fluorophores, light sources and filters at any point, or remove them by clicking X. The emission graph profiles will change in real time as the line is dragged to various places on the horizontal axis, representing the intensity of response to an excitation source at that wavelength. To view a known dye, scroll down the menu on the left and click to select. Click one or more choices. To predict the response of the fluorophores on the graph to an excitation laser, click "Predict Intensity" under the "Analyze" submenu. Spectra Viewer and Experiment Design. To remove the filter from the workspace entirely, click the "x" to the right of the filter title. Click "Disable Intensity Prediction" to return to the default visual display. The light source and filters for the chosen instrument will be displayed automatically. Cart 0 Sign In. To change the current selection, click a different dye name. The choice currently selected will be highlighted in blue. To change the horizontal display axis, click "Set Axis". A popup box will appear on the top of the page with an input box for the starting wavelength. The email includes an automatically-assigned ID code which corresponds to your selections, which can be retrieved and loaded to the SpectraViewer by clicking Load and entering the ID code.

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Alternatively, you can choose Custom Instrument or make no selection and add the light sources and filters manually. To use the viewer for absorbance dyes colorimetric format labels , click the left drop-down menu under "Current mode" and select "Absorbance". The choice currently selected will be highlighted in blue. Click one or more choices. The user will then be prompted to enter an ending wavelength value. Analyzing Spectra [ Show ] Click "Show Crosshairs" under the "Analyze" submenu in order to trace the exact curve of the currently-selected compounds" fluorescent intensity across the horizontal axis. Adjusting Graph Display [ Show ] If a selected compound is no longer of interest, click the checkbox to the right of the compound name as displayed on the bottom of the page to deselect it. Click here to see all available distributors. To remove the filter from the workspace entirely, click the "x" to the right of the filter title. To remove a filter from showing on the graph, deselect the check box to the right of the filter title. Email Required. The Spectrum Viewer is set to fluorescent mode by default.